Don’t think so. I’ve never experienced it when I was playing around a lot with locking and unlocking and I’ve read a lot of reports from users having locked the bootloader (after /e/OS installation) and getting an unbootable device but being able to unlock it again (because OEM unlock had been enabled).
I also can’t remember any report where OEM unlock was enabled and (only) locking the device changed this.
(Unfortunately I don’t have a test device atm to try it out myself.)
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